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power tm primary hep medium  (TaKaRa)


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    TaKaRa power tm primary hep medium
    Power Tm Primary Hep Medium, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/power+primary+hep+medium/Cellartis+Power+Primary+HEP+Medium/bio_rxiv__64898__2026__04__21__719874-330-11-18
    Average 94 stars, based on 16 article reviews
    power tm primary hep medium - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Sequence based prediction of cell type specific microRNA binding and mRNA degradation for therapeutic discovery
    Article Snippet: These were screened using an AlphaLISA assay [PerkinElmer: AL389C] to ascertain PON1 protein expression within primary human hepatocyte (PHH) cells. .. 18,000 PHH cells per well were reverse transfected with ASO using RNAiMAX [Thermofisher: 13778-150] in a 384-well format in InVitroGRO CP Hepatocyte Medium (BioIVT: Z99029) supplemented with ROCK inhibitor-Y-27632 [Tocris Small Molecules: 1254/1] for 24 hours, the media was then changed to Cellartis Power Primary HEP Medium (Takara Bioscience: Y20020) until day 7. .. The cells were then lysed in Alphalisa lysis buffer [PerkinElmer: AL003F] supplemented with 1X HALT protease buffer [Thermofisher: 78439] and samples were analyzed using the PON1 (human) AlphaLISA Detection Kit [PerkinElmer: AL389C].

    Article Title: A novel bispecific siRNA concept: Efficient dual knockdown of YAP1 and WWTR1 with a single guide strand
    Article Snippet: Human hepatocyte HepaSH cells (Central Institute for Experimental Medicine and Life Science, Kanagawa, Japan, FSSF001, 25-2423) were seeded at 1 × 10 6 cells/well in 96-well collagen I-coated plates (Corning, Corning, NY, USA, 356407) in culture medium (MIL222) according to the manufacturer’s instructions. .. After 24 h, the medium was replaced with Cellartis Power Primary HEP Medium (Takara Bio, Y20020), and then the siRNAs were transfected using Lipofectamine RNAiMAX according to the manufacturer’s instructions. ..

    Allele-specific Oligonucleotide:

    Article Title: Sequence based prediction of cell type specific microRNA binding and mRNA degradation for therapeutic discovery
    Article Snippet: These were screened using an AlphaLISA assay [PerkinElmer: AL389C] to ascertain PON1 protein expression within primary human hepatocyte (PHH) cells. .. 18,000 PHH cells per well were reverse transfected with ASO using RNAiMAX [Thermofisher: 13778-150] in a 384-well format in InVitroGRO CP Hepatocyte Medium (BioIVT: Z99029) supplemented with ROCK inhibitor-Y-27632 [Tocris Small Molecules: 1254/1] for 24 hours, the media was then changed to Cellartis Power Primary HEP Medium (Takara Bioscience: Y20020) until day 7. .. The cells were then lysed in Alphalisa lysis buffer [PerkinElmer: AL003F] supplemented with 1X HALT protease buffer [Thermofisher: 78439] and samples were analyzed using the PON1 (human) AlphaLISA Detection Kit [PerkinElmer: AL389C].

    Cell Culture:

    Article Title: Suppression of HBV replication and expression by CRISPR/Cas9 ribonucleoproteins.
    Article Snippet: HBV infection is a global public health problem.. The current treatment using nucleotide analogues (NA) can suppress viral replication but cannot eliminate HBV infection due to the persistence of covalently closed circular DNA (cccDNA), which sustains HBV replication and integration into the host cell genome and is refractory to NA treatment.. CRISPR/Cas9 has been used to disrupt integrated HBV DNA and minichromosomal cccDNA for HBV suppression, but its expression and delivery require viral or non-viral vectors, which pose safety concerns for human application.

    Article Title: A hnRNPA2B1 agonist effectively inhibits HBV and SARS-CoV-2 omicron in vivo
    Article Snippet: HEK293T and HepG2 cells were purchased from ATCC and cultured in high-glucose DMEM supplemented with 10% FBS. .. Primary human hepatocytes isolated from normal liver tissue and cultured with Power Primary HEP medium (Takara, San Francisco, CA) in the plates coated with 50 μg/mL of rat tail collagen type I (Sigma-Aldrich, St. Louis, MO). ..

    Article Title: Suppression of HBV replication and expression by CRISPR/Cas9 ribonucleoproteins
    Article Snippet: .. The cells were cultured using Cellartis Power Primary Hep Medium (Cat# Y20020, Takara Bio, San Jose, CA) and infected with HBV using a spinoculation method ( Yan et al., 2015 ). ..

    Infection:

    Article Title: Suppression of HBV replication and expression by CRISPR/Cas9 ribonucleoproteins.
    Article Snippet: HBV infection is a global public health problem.. The current treatment using nucleotide analogues (NA) can suppress viral replication but cannot eliminate HBV infection due to the persistence of covalently closed circular DNA (cccDNA), which sustains HBV replication and integration into the host cell genome and is refractory to NA treatment.. CRISPR/Cas9 has been used to disrupt integrated HBV DNA and minichromosomal cccDNA for HBV suppression, but its expression and delivery require viral or non-viral vectors, which pose safety concerns for human application.

    Article Title: Suppression of HBV replication and expression by CRISPR/Cas9 ribonucleoproteins
    Article Snippet: .. The cells were cultured using Cellartis Power Primary Hep Medium (Cat# Y20020, Takara Bio, San Jose, CA) and infected with HBV using a spinoculation method ( Yan et al., 2015 ). ..

    Isolation:

    Article Title: A hnRNPA2B1 agonist effectively inhibits HBV and SARS-CoV-2 omicron in vivo
    Article Snippet: HEK293T and HepG2 cells were purchased from ATCC and cultured in high-glucose DMEM supplemented with 10% FBS. .. Primary human hepatocytes isolated from normal liver tissue and cultured with Power Primary HEP medium (Takara, San Francisco, CA) in the plates coated with 50 μg/mL of rat tail collagen type I (Sigma-Aldrich, St. Louis, MO). ..

    other:

    Article Title: Expandable, Functional Hepatocytes Derived from Primary Cells Enable Liver Therapeutics
    Article Snippet: Culture media was removed, and Cellartis® PowerTM Primary HEP Medium (Takara) was added to the cells and cultured for an additional 72 hours, with media changed every day.



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    power tm primary hep medium  (TaKaRa)
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    TaKaRa power tm primary hep medium
    Power Tm Primary Hep Medium, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cellartis power hepatocyte medium  (TaKaRa)
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    Efficient transgene insertion in PHHs requires homology. PHHs were thawed, plated, and cultured overnight prior to being treated with LNPs containing 500 ng/1e6 cells of mRNA encoding a HAO1 -specific ARCUS nuclease and 1e 5 v.g./cell of an AAV6 vector encoding a droplet digital PCR amplicon. Seven days after editing, DNA was harvested and HAO1 disruption and transgene insertion were measured by ddPCR. The frequency of disrupted HAO1 alleles (sum of small indels plus AAV gene insertions in light green, and AAV insertions in dark green) is plotted in panel ( A ). Repair outcomes were determined by Nanopore long read sequencing and the results appear in panel ( B ). Alternatively, PHHs were edited and transduced with an AAV6 vector encoding a P2A-furin controlled GFP cassette. Cells were analyzed by flow cytometry (panels C – F ), to quantify AAV insertions. Significance was determined using ANOVA and a Tukey–Kramer multiple comparisons correction. Data are representative of three biological replicates using <t>hepatocytes</t> from two different donors; * P < 0.05, ** P < 0.01, n = 3. Edited PHHs were also transduced with AAV6 vectors encoding HA variants (both HAs, no homology, microhomology, left arm only, right arm only) and insertion was measured by flow cytometry ( G – K ) or ddPCR ( L ).
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    powertm primary hep medium  (TaKaRa)
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    Efficient transgene insertion in PHHs requires homology. PHHs were thawed, plated, and cultured overnight prior to being treated with LNPs containing 500 ng/1e6 cells of mRNA encoding a HAO1 -specific ARCUS nuclease and 1e 5 v.g./cell of an AAV6 vector encoding a droplet digital PCR amplicon. Seven days after editing, DNA was harvested and HAO1 disruption and transgene insertion were measured by ddPCR. The frequency of disrupted HAO1 alleles (sum of small indels plus AAV gene insertions in light green, and AAV insertions in dark green) is plotted in panel ( A ). Repair outcomes were determined by Nanopore long read sequencing and the results appear in panel ( B ). Alternatively, PHHs were edited and transduced with an AAV6 vector encoding a P2A-furin controlled GFP cassette. Cells were analyzed by flow cytometry (panels C – F ), to quantify AAV insertions. Significance was determined using ANOVA and a Tukey–Kramer multiple comparisons correction. Data are representative of three biological replicates using <t>hepatocytes</t> from two different donors; * P < 0.05, ** P < 0.01, n = 3. Edited PHHs were also transduced with AAV6 vectors encoding HA variants (both HAs, no homology, microhomology, left arm only, right arm only) and insertion was measured by flow cytometry ( G – K ) or ddPCR ( L ).
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    Image Search Results


    Efficient transgene insertion in PHHs requires homology. PHHs were thawed, plated, and cultured overnight prior to being treated with LNPs containing 500 ng/1e6 cells of mRNA encoding a HAO1 -specific ARCUS nuclease and 1e 5 v.g./cell of an AAV6 vector encoding a droplet digital PCR amplicon. Seven days after editing, DNA was harvested and HAO1 disruption and transgene insertion were measured by ddPCR. The frequency of disrupted HAO1 alleles (sum of small indels plus AAV gene insertions in light green, and AAV insertions in dark green) is plotted in panel ( A ). Repair outcomes were determined by Nanopore long read sequencing and the results appear in panel ( B ). Alternatively, PHHs were edited and transduced with an AAV6 vector encoding a P2A-furin controlled GFP cassette. Cells were analyzed by flow cytometry (panels C – F ), to quantify AAV insertions. Significance was determined using ANOVA and a Tukey–Kramer multiple comparisons correction. Data are representative of three biological replicates using hepatocytes from two different donors; * P < 0.05, ** P < 0.01, n = 3. Edited PHHs were also transduced with AAV6 vectors encoding HA variants (both HAs, no homology, microhomology, left arm only, right arm only) and insertion was measured by flow cytometry ( G – K ) or ddPCR ( L ).

    Journal: Nucleic Acids Research

    Article Title: High-efficiency homology-directed insertion into the genome using the engineered homing endonuclease ARCUS

    doi: 10.1093/nar/gkaf961

    Figure Lengend Snippet: Efficient transgene insertion in PHHs requires homology. PHHs were thawed, plated, and cultured overnight prior to being treated with LNPs containing 500 ng/1e6 cells of mRNA encoding a HAO1 -specific ARCUS nuclease and 1e 5 v.g./cell of an AAV6 vector encoding a droplet digital PCR amplicon. Seven days after editing, DNA was harvested and HAO1 disruption and transgene insertion were measured by ddPCR. The frequency of disrupted HAO1 alleles (sum of small indels plus AAV gene insertions in light green, and AAV insertions in dark green) is plotted in panel ( A ). Repair outcomes were determined by Nanopore long read sequencing and the results appear in panel ( B ). Alternatively, PHHs were edited and transduced with an AAV6 vector encoding a P2A-furin controlled GFP cassette. Cells were analyzed by flow cytometry (panels C – F ), to quantify AAV insertions. Significance was determined using ANOVA and a Tukey–Kramer multiple comparisons correction. Data are representative of three biological replicates using hepatocytes from two different donors; * P < 0.05, ** P < 0.01, n = 3. Edited PHHs were also transduced with AAV6 vectors encoding HA variants (both HAs, no homology, microhomology, left arm only, right arm only) and insertion was measured by flow cytometry ( G – K ) or ddPCR ( L ).

    Article Snippet: PHHs were sourced from BioIVT (Westbury, NY) and were cultured on Corning BioCoat collagen plates (Cat# 326408, Corning, NY) INVITROGRO CP medium (BioIVT, Z99209 ) supplemented with TORPEDO antibiotics (BioIVT, Z99000 ) or Cellartis Power Hepatocyte medium (Takara Bio, San Jose, CA, Cat# Y20020).

    Techniques: Cell Culture, Plasmid Preparation, Digital PCR, Amplification, Disruption, Sequencing, Transduction, Flow Cytometry

    SDSA and 3′ overhangs drive transgene insertion in PHHs. PHHs were thawed, plated, and cultured overnight prior to being treated with LNPs containing mRNA encoding a HAO1 -specific ARCUS nuclease and an AAV6 vector encoding a P2A-furin controlled GFP cassette. Editing was carried out in the presence of vehicle alone, 500 μM TDRL-551, 90 μM zelpolib, or 200 μM 6-hydroxy dopamine. Seven days following editing, cells were analyzed for GFP frequency via flow cytometry. Representative GFP histograms are shown in panels ( A – D ) and the means of three technical replicates appear in panel ( E ). Significance was determined using ANOVA and a Tukey–Kramer multiple comparisons correction. Data are representative of three biological replicates using hepatocytes from two different donors; ** P < 0.01, n = 3. PHHs were also treated with LNPs carrying either mRNA encoding a HAO1 -specific ARCUS nuclease or the same nuclease linked to a TREX1 domain. Seven days later GFP frequencies were measured by flow cytometry and the results are plotted in panel ( F ). Significance was assessed using a two-tailed Student’s t- test; ** P < 0.013, n = 3.

    Journal: Nucleic Acids Research

    Article Title: High-efficiency homology-directed insertion into the genome using the engineered homing endonuclease ARCUS

    doi: 10.1093/nar/gkaf961

    Figure Lengend Snippet: SDSA and 3′ overhangs drive transgene insertion in PHHs. PHHs were thawed, plated, and cultured overnight prior to being treated with LNPs containing mRNA encoding a HAO1 -specific ARCUS nuclease and an AAV6 vector encoding a P2A-furin controlled GFP cassette. Editing was carried out in the presence of vehicle alone, 500 μM TDRL-551, 90 μM zelpolib, or 200 μM 6-hydroxy dopamine. Seven days following editing, cells were analyzed for GFP frequency via flow cytometry. Representative GFP histograms are shown in panels ( A – D ) and the means of three technical replicates appear in panel ( E ). Significance was determined using ANOVA and a Tukey–Kramer multiple comparisons correction. Data are representative of three biological replicates using hepatocytes from two different donors; ** P < 0.01, n = 3. PHHs were also treated with LNPs carrying either mRNA encoding a HAO1 -specific ARCUS nuclease or the same nuclease linked to a TREX1 domain. Seven days later GFP frequencies were measured by flow cytometry and the results are plotted in panel ( F ). Significance was assessed using a two-tailed Student’s t- test; ** P < 0.013, n = 3.

    Article Snippet: PHHs were sourced from BioIVT (Westbury, NY) and were cultured on Corning BioCoat collagen plates (Cat# 326408, Corning, NY) INVITROGRO CP medium (BioIVT, Z99209 ) supplemented with TORPEDO antibiotics (BioIVT, Z99000 ) or Cellartis Power Hepatocyte medium (Takara Bio, San Jose, CA, Cat# Y20020).

    Techniques: Cell Culture, Plasmid Preparation, Flow Cytometry, Two Tailed Test